Simultaneous selection and cloning of transfected eukaryotic cells.
نویسندگان
چکیده
منابع مشابه
Cloning and Expression of VP2 Gene of Infectious Bursal Disease Virus in Eukaryotic Cells
Infectious bursal disease (IBD) is an economically important viral disease of chickens with worldwide distribution which suppresses the immune system of young chickens. VP2 is the major host-protective protein of infectious bursal disease virus (IBDV). The encoding region of VP2 protein was PCR amplified from a plasmid containing a cDNA fragment of large genomic segment of IBDV, strain D78. Thi...
متن کاملCloning and secretory expression of VP2 gene of infectious bursal disease virus in eukaryotic cells
VP2 gene coding region of a vaccinal strain (D78) of infectious bursal disease virus (IBDV) was clonedin a eukaryotic expression vector, pSec Tag2A. The gene was placed downstream of Ig κ chain leadersequence, under the control of human cytomegalovirus (hCMV) immediate early enhancer and promoter. Theconstruct pSec Tag2A-VP2 was transfected in COS-7 cell line and the expression and secretion of...
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Background: Tuberculosis (TB) remains as a major cause of death around the world. Construction of a new vaccine against tuberculosis is an effective way to control it. Several vaccines against this disease have been developed. The aim of the present study was to cloning of tb10.4 gene in pcDNA3.1+ plasmid and evaluation of its expression in eukaryotic cells. ...
متن کاملDesign and Cloning of the Optimized L1 Gene from Human Papilloma virus 18 into the Expression Vector PcDNA3 and Evaluating its Expression in a Eukaryotic System
Background: Vaccines have played a special role in controlling and reducing mortality from infectious diseases. In this regard, DNA vaccines were developed to ease the production and reduce the risks of traditional vaccines. Human papillomavirus (HPV) has been introduced as the causing agent of cervical cancer. The capsid protein (L1) of HPV has been used to produce subunit and DNA vaccines. Th...
متن کاملCloning and Expression of Rabies Virus Glycoprotein Gene into Eukaryotic System
Background and Aims: The aim of this study was cloning and expression of rabies virus glycoprotein by a eukaryotic expression plasmid pcDNA3.1(+) in BSR cell line. This construct might be used for a potential DNA vaccine. Materials and Methods: Glycoprotein gene was synthesized and cloned into pBluescript vector and then sub cloned into eukaryotic expression vector (pcDNA3.1(+)). After verifica...
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ورودعنوان ژورنال:
- BioTechniques
دوره 21 4 شماره
صفحات -
تاریخ انتشار 1996